Lab Resources

 Databases and protocols

Below are links to the lab database (plasmids, primers, cell stocks, etc) and to lab protocols.

Please reach out to wiedenheft_lab@montana.edu if you need access to these resources.

• Lab database
• Lab protocols

Computational Resources

• Lewis
• Tempest
• Github 

Bioinformatics

• Check back soon for tutorials on structure-guided HMM searches, tree-building, and neighborhood analysis.

CryoEM

Getting started with cryoEM in the Wiedenheft lab? Start here with these introductory videos:

• Gabe Lander's 3-minute introduction to cryoEM
• Grant Jensen's excellent introductory course
• Fred Sigworth's theoretical overview of cryoEM

Montana State University's cryoEM facility is up and running! Check out these facility resources:

• cryo-EM facility
• cryo-EM facility documentation

Wiedenheft lab resources:

• Ongoing EM projects (must be a lab member to access)
• Facility storage dewar spreadsheet (must be a lab member to access)

General Tutorials for cryoEM

• Negative stain microscopy
• Freezing and clipping
• Setting up a data collection
• Image processing - cryoSPARC
  • Email Will Henriques or tempest-support@montana.edu to get set up on cryoSPARC
• Relion tutorial
• Model building tutorials